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Grape seed extract proanthocyanidins downregulate HIV-1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells
Nair, Madhavan P; Kandaswami, Chithan; Mahajan, Supriya; Nair, Harikrishna N; Chawda, Ram; Shanahan, Thomas; Schwartz, Stanley A.
  • Nair, Madhavan P; Buffalo General Hospital. Kaleida Health System. Departments of Medicine and Microbiology. US
  • Kandaswami, Chithan; State University of New York at Buffalo. Department of Clinical Laboratory and Biotechnical Sciences. US
  • Mahajan, Supriya; Buffalo General Hospital. Kaleida Health System. Departments of Medicine and Microbiology. US
  • Nair, Harikrishna N; Buffalo General Hospital. Kaleida Health System. Departments of Medicine and Microbiology. US
  • Chawda, Ram; Buffalo General Hospital. Kaleida Health System. Departments of Medicine and Microbiology. US
  • Shanahan, Thomas; Buffalo General Hospital. Kaleida Health System. Departments of Medicine and Microbiology. US
  • Schwartz, Stanley A; Buffalo General Hospital. Kaleida Health System. Departments of Medicine and Microbiology. US
Biol. Res ; 35(3/4): 421-431, 2002. ilus, graf
Artigo em Inglês | LILACS | ID: lil-339735
RESUMO
Flavonoids and related polyphenols, in addition to their cardioprotective, anti-tumor, anti-inflammatory, anti-carcinogenic and anti-allergic activities, also possess promising anti-HIV effects. Recent studies documented that the ß-chemokine receptors, CCR2b, CCR3 and CCR5, and the a-chemokine receptors, CXCR1, CXCR2 and CXCR4 serve as entry coreceptors for HIV-1. Although flavonoids and polyphenolic compounds elicit anti-HIV effects such as inhibition of HIV-1 expression and virus replication, the molecular mechanisms underlying these effects remain to be clearly elucidated. We hypothesize that flavonoids exert their anti-HIV effects, possibly by interfering at the HIV co-receptor level. We investigated the effect of flavonoid constituents of a proprietary grape seed extract (GSE) on the expression of HIV-1 coentry receptors by immunocompetent mononuclear leukocytes. Our results showed that GSE significantly downregulated the expression of the HIV-1 entry co-receptors, CCR2b , CCR3 and CCR5 in normal PBMC in a dose dependent manner. Further , GSE treated cultures showed significantly lower number of CCR3 positive cells as quantitated by flow cytometry analysis which supports RT-PCR gene expression data.Investigations of the mechanisms underlying the anti-HIV-1 effects of grape seed extracts may help to identify promising natural products useful in the prevention and /or amelioration of HIV-1 infection
Assuntos
Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Leucócitos Mononucleares / HIV-1 / Receptores de Quimiocinas / Vitis / Antocianinas / Antioxidantes Limite: Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2002 Tipo de documento: Artigo / Documento de projeto País de afiliação: Estados Unidos Instituição/País de afiliação: Buffalo General Hospital/US / State University of New York at Buffalo/US

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Leucócitos Mononucleares / HIV-1 / Receptores de Quimiocinas / Vitis / Antocianinas / Antioxidantes Limite: Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2002 Tipo de documento: Artigo / Documento de projeto País de afiliação: Estados Unidos Instituição/País de afiliação: Buffalo General Hospital/US / State University of New York at Buffalo/US