Distribution of versican and hyaluronan in the mouse uterus during decidualization
Braz. j. med. biol. res
;
36(8): 1067-1071, Aug. 2003. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-340780
ABSTRACT
Preparation for embryo implantation requires extensive adaptation of the uterine microenvironment. This process consists of cell proliferation and cell differentiation resulting in the transformation of endometrial fibroblasts into a new type of cell called decidual cell. In the present study, we followed the space-time distribution of versican and hyaluronan (HA) in different tissues of the uterus before and after embryo implantation. Fragments of mouse uteri obtained on the fourth, fifth, sixth and seventh days of pregnancy were fixed in Methacarn, embedded in Paraplast and cut into 5-æm thick sections. HA was detected using a biotinylated fragment of the proteoglycan aggrecan, which binds to this glycosaminoglycan with high affinity and specificity. Versican was detected by a polyclonal antibody. Both reactions were developed by peroxidase methods. Before embryo implantation, both HA and versican were present in the endometrial stroma. However, after embryo implantation, HA disappeared from the decidual region immediately surrounding the implantation chamber, whereas versican accumulated in the same region. The differences observed in the expression of HA and versican suggest that both molecules may participate in the process of endometrial decidualization and/or embryo implantation
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Útero
/
Blastocisto
/
Transferência Embrionária
Limite:
Animais
/
Gravidez
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2003
Tipo de documento:
Artigo
/
Congresso e conferência
/
Documento de projeto
País de afiliação:
Brasil
/
Chile
Instituição/País de afiliação:
Universidad de Santiago de Chile/CL
/
Universidade de Säo Paulo/BR
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