Epidemiología molecular de un brote de infecciones por streptococucus pyogenes en una unidad de quemados / Molecular epidemiology of a streptococcus pyogenes related nosocomial outbreak in a burn unit
Rev. méd. Chile
;
131(2): 145-154, 2003. tab, graf
Artigo
em Espanhol
| LILACS
| ID: lil-342235
RESUMO
Group A Streptococcal (GAS) infections have increased in frequency and severity worldwide. During April 1996, a nosocomial outbreak associated to GAS infections affected seven patients admitted to a pediatric burn unit. The causative organism was likely disseminated from the source patient to another child in the emergency room before he was transferred to the burn unit. Patients developed burn infections or invasive disease. One of them died due to a toxic shock syndrome and 3 other lost their skin grafts. Perineal and nasal microbiological surveillance of 42 related health care workers identified only one of them as carrier of S pyogenes. Aim:
To report a molecular analysis of an apparently clonal outbreak. Material andmethods:
The available isolates were analyzed by molecular methods including random amplified polymorphic DNA analysis (RAPD) with 4 different primers, Sma-I pulsed field gel electrophoresis (PFGE) analysis, and speA, speB and speC detection by polymerase chain reaction (PCR).Results:
Two phylogenetically distant and sequentially isolated bacterial groups were identified either by RAPD analysis with selected primers or by Smal-PFGE analysis. The first group involved isolates identified in two patients that included the lethal case. The second bacterial group comprised 5 clinical isolates and the perineal and nasal isolates obtained from a health care worker. Only strains belonging to the first group harbored the speA gene and were associated with invasive disease. The second group could be split further in two subgroups according to their speB profile.Conclusions:
RAPD analysis with selected primers can reproduce the PFGE-discriminating ability on the epidemiological analysis of GAS infections
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Infecções Estreptocócicas
/
Streptococcus pyogenes
/
Unidades de Queimados
/
Queimaduras
Tipo de estudo:
Estudo de rastreamento
Limite:
Humanos
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2003
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
Universidad de Chile/CL
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