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Improvement of xylanase production by a parasexual cross between Aspergillus niger strains
Loera, Octavio; Córdova, Jesús.
  • Loera, Octavio; Universidad Autónoma Metropolitana. Departamento de Biotecnología. México. MX
  • Córdova, Jesús; Universidad de Guadalajara. Departamento de Ingeniería Química. Guadalajara. MX
Braz. arch. biol. technol ; 46(2): 177-181, Mar. 2003. graf
Artigo em Inglês | LILACS | ID: lil-351390
ABSTRACT
A diploid strain (D4) isolated via parasexual recombination between two Aspergillus niger xylanase overproducing mutants was characterised in terms of enzyme production and catabolite repression by glucose. This strain increased xylanase production (607 nkat/ml), which was nearly 100 percent higher than titers achieved by the wild type strain (305 nkat/ml) and 28 percent higher than the best mutant used to induce parasexual cycle. Diploid D4 was also less sensitive to carbon catabolite repression by glucose, since xylanolytic activity was detected under conditions normally repressing production by the wild type strain. No decrease in maximal xylanase levels was observed in the presence of glucose for diploid D4
Texto completo: DisponíveL Índice: LILACS (Américas) Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2003 Tipo de documento: Artigo País de afiliação: México Instituição/País de afiliação: Universidad Autónoma Metropolitana/MX / Universidad de Guadalajara/MX

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Texto completo: DisponíveL Índice: LILACS (Américas) Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2003 Tipo de documento: Artigo País de afiliação: México Instituição/País de afiliação: Universidad Autónoma Metropolitana/MX / Universidad de Guadalajara/MX