Histochemical study of the extracellular matrix components in the follicular wall of induced polycystic ovaries
Braz. j. morphol. sci
;
20(2): 93-100, May-Aug. 2003. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-355089
RESUMO
Numerous models have been developed to study polycystic ovarian syndrome in rats. In the present study, the syndrome was induced by exposure to constant light. The histological structure and differential distribution of extracellular matrix (ECM) fibers as well as the glycosaminoglycans (GAGs) content and composition of the ovarian follicular wall of rats with polycystic syndrome were evaluated. Histochemical differences were observed in the graunlosa and theca externa of follicular cysts when compared to normal preovulatory follicles. The colagen content of the theca externa of follicular cysts, quantified by the picrosirius method, was higher than in the controls. The neural carbohydrate and acidic GAC levels were lower in the granulosa and higher in the theca externa of cyst follicles than in control ovaries. Histomorphometrically, the follicular diameter was both a convenient and appropriate measurement for describing the cyst status; there were no differences in the thickness of each follicular layer. In conclusion, differences in the components of ECM were observed in the follicular wall of ovarian cysts compared eith normal preovulatory follicles. Howere, sinde these changes did not occur uniformly in all layers of the follicular wall, their role in cyst development remains to be established.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Síndrome do Ovário Policístico
/
Matriz Extracelular
/
Histocitoquímica
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. morphol. sci
Assunto da revista:
Anatomia
Ano de publicação:
2003
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Center for Experimental Biology and Laboratory Animal Sciences/AR
/
Department of Histology and Embryology/AR
/
National University of La Plata/AR
/
National University of Litoral/AR
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