Hypotrophy of conduit artery walls of the offspring of nitric oxide-defective rats
Braz. j. med. biol. res
;
37(4): 601-606, Apr. 2004. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-357100
RESUMO
The objective of the present study was to investigate the structure of the arterial walls of the offspring stemming from nitric oxide (NO)-defective hypertensive parents. The parents were treated with N G-nitro-L-arginine methyl ester (40 mg kg-1 day-1) for 5 weeks. Blood pressure was measured noninvasively in six 30-day-old rats and nine age-matched controls. The cardiovascular system was perfused with glutaraldehyde at 120 mmHg. The thoracic aorta and carotid artery were processed for electron microscopy, and geometry was determined by light microscopy. Endothelial cells, smooth muscle cells (SMC) and extracellular matrix (ECM) were determined by the point counting method in electron micrographs of the carotid artery. The blood pressure of experimental offspring was 150.0 ± 2.3 vs 104.6 ± 2.1 mmHg (P < 0.01) for the controls and their heart/body weight ratio of 3.9 ± 0.1 vs 4.4 ± 0.2 (P < 0.05) for the controls indicated cardiac hypotrophy. The wall thickness (tunica intima and media) of the thoracic aorta and carotid artery of experimental offspring was decreased to 78.9 percent (P < 0.01) and 83.8 percent (P < 0.01), respectively, compared to controls, as confirmed by a respective cross-sectional area of 85.3 percent (P < 0.01) and 84.1 percent (P < 0.01). The wall thickness/inner diameter ratio was reduced to 75 percent (P < 0.01) in the thoracic artery and to 81.5 percent (P < 0.01) in the carotid artery. No change in endothelial cell volume density or ECM was observed in the tunica intima of the carotid artery, and SMC volume density was lower in the tunica media (37.6 ± 0.9 vs 44.7 ± 1.1 percent for controls, P < 0.01), indicating compromised SMC development. Interference with arginine metabolism, a decrease in NO, and other factors are possible mechanisms underlying the structural alterations of the cardiovascular system of offspring from NO-defective hypertensive rats.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Aorta Torácica
/
Artérias Carótidas
/
Óxido Nítrico Sintase
/
NG-Nitroarginina Metil Éster
/
Inibidores Enzimáticos
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2004
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Eslováquia
Instituição/País de afiliação:
Slovak Academy of Sciences/SK
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