CENP-B is a conserved gene among vegetal species
Genet. mol. res. (Online)
;
1(3): 241-245, Sept. 2002. ilus
Artigo
em Inglês
| LILACS
| ID: lil-357426
ABSTRACT
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a complete human CENP-B gene. In all the plant species, anti-CENP-B antibodies recognized an 80-kDa protein. A 360-bp sequence encoding for the amino terminus region of the CENP-B protein was amplified by PCR in all the species and the nick translated pG/CNPB probe hybridized with the PCR products. Apparently the CENP-B centromere protein or an equivalent protein is widely distributed in the vegetal kingdom.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Verduras
/
Proteínas Cromossômicas não Histona
Limite:
Humanos
Idioma:
Inglês
Revista:
Genet. mol. res. (Online)
Assunto da revista:
Biologia Molecular
/
Genética
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
México
Instituição/País de afiliação:
CBE Universidad Autónoma de Zacatecas/MX
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