Reactive oxygen species inactivation improves pancreatic capillary blood flow in caerulein-induced pancreatitis in rats

ABSTRACT

Purpose: Reactive oxygen species (ROS) inactivation was studied to determine alterations in the pancreatic capillary blood flow (PCBF) during caerulein-induced pancreatitis in rats. Methods: A laser-Doppler flowmeter to measure PCBF and N-t-Butyl-Phenylnitrone (PBN) compound to inactivate ROS were used. Forty rats were divided in groups 1) control; 2) caerulein; 3) PBN; 4) caerulein+PBN. Serem biochemistry and histopathological analyses were performed. Results: PCBF measured a mean of 109.08 ± 14.54 percent, 68.24 t 10.47 percent, 102.18 ± 10.23 percent and 87.73 ± 18.72 percent in groups 1, 2, 3 and 4, respectively. PCBF in groups 2 and 4 decreased 31.75 ± 16.79 percent and 12.26 ± 15.24 percent, respectively. Serum amylase was 1323.70 ± 239.10 U/l, 2184.60 ± 700.46 U/1, 1379.80 t 265.72 U/1 and 1622.10 ± 314.60 U/1 in groups 1, 2, 3 and 4, respectively. There was a significant difference in the PCBF and serem amylase when compared groups 2 and 4. Cytoplasmatic vacuolation was present in groups 2 and 4. Otherwise, no qualitative changes were seen. Conclusion: ROS inactivation improves PCBF and minimizes the serem amylase increase during caerulein-induced pancreatitis. ROS effect may be one of the leading causative events in this model of acute pancreatitis.