Your browser doesn't support javascript.
loading
The Shiga toxin 2 B subunit inhibits net fluid absorption in human colon and elicits fluid accumulation in rat colon loops
Creydt, V. Pistone; Miyakawa, M. Fernandez; Martín, F; Zotta, E; Silberstein, C; Ibarra, C.
  • Creydt, V. Pistone; Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires. AR
  • Miyakawa, M. Fernandez; Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires. AR
  • Martín, F; Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires. AR
  • Zotta, E; Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires. AR
  • Silberstein, C; Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires. AR
  • Ibarra, C; Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires. AR
Braz. j. med. biol. res ; 37(6): 799-808, Jun. 2004. ilus, tab, graf
Artigo em Inglês | LILACS | ID: lil-359907
RESUMO
Shiga toxin (Stx)-producing Escherichia coli (STEC) colonizes the large intestine causing a spectrum of disorders, including watery diarrhea, bloody diarrhea (hemorrhagic colitis), and hemolytic-uremic syndrome. It is estimated that hemolytic-uremic syndrome is the most common cause of acute renal failure in infants in Argentina. Stx is a multimeric toxin composed of one A subunit and five B subunits. In this study we demonstrate that the Stx2 B subunit inhibits the water absorption (Jw) across the human and rat colonic mucosa without altering the electrical parameters measured as transepithelial potential difference and short circuit current. The time-course Jw inhibition by 400 ng/ml purified Stx2 B subunit was similar to that obtained using 12 ng/ml Stx2 holotoxin suggesting that both, A and B subunits of Stx2 contributed to inhibit the Jw. Moreover, non-hemorrhagic fluid accumulation was observed in rat colon loops after 16 h of treatment with 3 and 30 ng/ml Stx2 B subunit. These changes indicate that Stx2 B subunit induces fluid accumulation independently of A subunit activity by altering the usual balance of intestinal absorption and secretion toward net secretion. In conclusion, our results suggest that the Stx2 B subunit, which is non-toxic for Vero cells, may contribute to the watery diarrhea observed in STEC infection. Further studies will be necessary to determine whether the toxicity of Stx2 B subunit may have pathogenic consequences when it is used as a component in an acellular STEC vaccine or as a vector in cancer vaccines.
Assuntos
Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Toxinas Bacterianas / Água / Transporte de Íons / Colo / Escherichia coli / Mucosa Intestinal Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2004 Tipo de documento: Artigo / Documento de projeto País de afiliação: Argentina Instituição/País de afiliação: Universidad de Buenos Aires/AR

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Toxinas Bacterianas / Água / Transporte de Íons / Colo / Escherichia coli / Mucosa Intestinal Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2004 Tipo de documento: Artigo / Documento de projeto País de afiliação: Argentina Instituição/País de afiliação: Universidad de Buenos Aires/AR