A rapid polymerase chain reaction protocol to detect adenovirus in eye swabs
Arq. bras. oftalmol
;
67(3): 423-427, maio-jun. 2004. ilus
Artigo
em Inglês
| LILACS
| ID: lil-361716
ABSTRACT
Purpose:
Viruses of the Adenoviridae family are associated with many clinical syndromes, processing 50 serotypes. These agents and viruses of the Herpesviridae family are the two major agents responsible for viral conjunctivitis, and a rapid diagnosis is important due to the epidemic character of adenoviral infection.Methods:
We developed a PCR without DNA extraction for adenovirus using primers thath amplify a 300 tp gragament of the hexon capsid protein gene from many serotypes.Results:
Swab samples from cornea of seven patients with keratoconjunctivitis were analyzed, and one of them was PCR positive for adenovirus. The sequence of this fragment shows a 100 percent homology with the sequence of adenovirus type 8.Conclusion:
Sequencing of 300 bp from the hexon gene allows to identity almost all Ad serotypes, including all serotypes related to epidemic keratokonjuntivitis (8, 19,37) and almost all serotypes involved with Ad-associated conjunctivitis.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Adenovírus Humanos
/
Reação em Cadeia da Polimerase
/
Túnica Conjuntiva
/
Córnea
/
Ceratoconjuntivite
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Arq. bras. oftalmol
Assunto da revista:
Oftalmologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Núcleo Especializado em Oftalmologia/BR
/
Universidade Federal de Minas Gerais/BR
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