Use of small intestine submucosa as ureteral allograft in pigs
Int. braz. j. urol
;
30(4): 327-335, Jul.-Aug. 2004.
Artigo
em Inglês
| LILACS
| ID: lil-383752
RESUMO
PURPOSE:
The aim of the present study was to evaluate the biocompatibility of small intestine submucosa (SIS) in the reconstruction of the ureter in swine. MATERIALS ANDMETHODS:
An experimental study was performed in 10 half-breed pigs weighing between 20 and 30 K, in which a previously prepared segment of SIS measuring approximately 2.0 cm was implanted in the upper third part of the right ureter.RESULTS:
Of the 10 operated animals, one died 14 days after the surgery due to a dehiscence on the suture line of the implanted graft. The remaining 9 animals were submitted to ultrasound examination of the urinary tract and were sacrificed on the 40th postoperative day. The macroscopic evaluation showed no calculus, incrustation, fistula, abscesses or adhesions in the ureters with the graft. Microscopic evaluation with hematoxylin-eosin and Sirius red showed in the experimental area (graft) the presence of urothelium in 100 percent of the cases, collagen in 100 percent of the cases, and smooth muscle layer in 87.5 percent of the animals. In the area adjacent to the graft (proximal and distal), we observed 92.86 percent of urothelium, 42.86 percent of collagen and 71.43 percent of smooth muscle. In the contralateral ureter, it was found 100 percent of urothelium and smooth muscle and just 11.11 percent of collagen. The microscopic analysis of the kidneys whose ureters received the graft of SIS evidenced congestion in 55.55 percent, pelvic edema in 66.66 percent and interstitial nephritis in 77.78 percent. Hydronephrosis was present in 33.33 percent and chronic pyelonephritis in 44 percent. Only 1 animal presented total absence of glomerulus in the renal parenchyma.CONCLUSION:
The SIS graft behaved as a biological tissue support, allowing the regeneration of the urothelium and smooth muscle grow, despite of chronic inflammatory process.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Ureter
/
Mucosa Intestinal
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Int. braz. j. urol
Assunto da revista:
Urologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Pontifical Catholic University of Parana/BR
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