Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina
Biocell
;
27(3): 311-318, Dec. 2003.
Artigo
em Inglês
| LILACS
| ID: lil-384239
ABSTRACT
The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg x L(-1) benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned medium, but with 0.15 mg x L(-1) of BAP. After 2 months, microshoots with 2-3 leaves were selected and cultured in BTMm-agar, supplemented with 5 mg x L(-1) IBA or in liquid BTMm on perlite and, in the presence or absence of A. rhizogenes (Ar) and in combination with 3 mg x L(-1) IBA. Rooting in P. chilensis reached 100.0% when Ar infection was produced in the presence of IBA, increasing both, the number and dry weight of roots. In N. alpina, 90.0% of rooting efficiency was obtained when Ar infection was produced in liquid culture and in the absence of auxin.
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Índice:
LILACS (Américas)
Assunto principal:
Rhizobium
/
Árvores
/
Raízes de Plantas
/
Prosopis
/
Indução Embrionária
/
Ácidos Indolacéticos
País/Região como assunto:
América do Sul
/
Chile
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2003
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Universidad Nacional del Sur/AR
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