Ageing induces apoptosis and increases HSP70 stress protein in the epididymis of Octodon degus
Int. j. morphol
;
22(1): 29-34, 2004. ilus
Artigo
em Inglês
| LILACS
| ID: lil-384575
ABSTRACT
Apoptosis has been largely analyzed in the testis. Nevertheless, the epididymis has been scarcely studied. We analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the South American rodent Octodon degus both young and senile. Apoptosis was identified using the TUNEL method which detects in situ DNA fragmentation. Apoptosis was detected in the principal cells of the epididymal epithelium. The caput epididymis was the region more affected. The caput of young animals showed that 0.32/1000 cells were apoptotic in contrast to 5.1/1000 of senile animals. Also in the cauda epididymis apoptosis is increased with age, appearing 0.14/1000 and 3.9/1000 in young and senile animals, respectively. On the other hand, we used a immunocytochemical method to localize the stress protein HSP70. HSP70 appeared notably increased in the principal cells of the cauda epididymis of senile animals. Changes in the epididymal epithelium are probably due to the low androgen levels existing in senile animals and are a region dependent phenomenon
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Roedores
/
Senescência Celular
/
Apoptose
/
Proteínas de Choque Térmico HSP70
/
Epididimo
Limite:
Animais
Idioma:
Inglês
Revista:
Int. j. morphol
Assunto da revista:
Anatomia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Chile
/
Espanha
Instituição/País de afiliação:
Centro de Investigaciones Biológicas/ES
/
Universidad de Chile/CL
Similares
MEDLINE
...
LILACS
LIS