Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
Electron. j. biotechnol
;
7(2): 115-123, Aug. 2004. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-387552
ABSTRACT
The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Oligossacarídeos
/
Pichia
/
Gluconacetobacter
/
Frutose
/
Hexosiltransferases
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2004
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Cuba
Instituição/País de afiliação:
Centro de Ingeniería Genética y Biotecnológica/CU
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