Similarity between the association factor of ribosomal subunits and the protein Stm1p from Saccharomyces cerevisiae
Mem. Inst. Oswaldo Cruz
;
99(7): 733-737, Nov. 2004. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-391603
ABSTRACT
A ribosome association factor (AF) was isolated from the yeast Sacchharomyces cerevisiae. Partial amino acid sequence of AF was determined from its fragment of 25 kDa isolated by treating AF with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-Bromoindolenine (BNPS-skatole). This sequence has a 86 percent identity to the product of the single-copy S. cerevisiae STM1 gene that is apparently involved in several events like binding to quadruplex and triplex nucleic acids and participating in apoptosis, stability of telomere structures, cell cycle, and ribosomal function. Here we show that AF and Stm1p share some characteristics both bind to quadruplex and Pu triplex DNA, associates ribosomal subunits, and are thermostable. These observations suggest that these polypeptides belong to a family of proteins that may have roles in the translation process.
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Índice:
LILACS (Américas)
Assunto principal:
Saccharomyces cerevisiae
/
RNA Fúngico
/
RNA Ribossômico
/
Análise de Sequência de Proteína
Tipo de estudo:
Fatores de risco
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2004
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Estados Unidos
/
Venezuela
Instituição/País de afiliação:
Medical College of Wisconsin/US
/
Universidad de Carabobo/VE
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