Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques
Mem. Inst. Oswaldo Cruz
;
99(7): 749-752, Nov. 2004. ilus
Artigo
em Inglês
| LILACS
| ID: lil-391606
RESUMO
Transmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) and a problem in certain risk populations. Therefore, knowledge of resistance of M. bovis towards antibiotics used for therapy of human Tb could help avoiding cure delay and treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility of M. bovis isolates towards streptomycin, isoniazide, rifampicin, ethambutol, and ethionamide, the first line antibiotics for human Tb. Therefore, 185 clinical samples from cattle with clinical signs of tuberculosis were processed and submitted to culturing and bacterial isolates to identification and drug susceptibility testing using the proportion method. Among 89 mycobacterial strains, 65 were identified as M. bovis and none were resistant to any of the antibiotics used. Confirmation of present results by future studies, enrolling a large number of isolates and designed to properly represent Brazilian regions, may favor the idea of using isoniazide preventive therapy as part of a Tb control strategy in special situations. Also, nucleic acids from bacterial isolates were submitted to rifoligotyping, a recently described reverse hybridization assay for detection of mutations causing resistance towards rifampicin. Concordance between the conventional and the molecular test was 100 percent, demonstrating the use of such methodology for rapid evaluation of drug susceptibility in M. bovis.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Testes de Sensibilidade Microbiana
/
Mycobacterium bovis
/
Antituberculosos
Tipo de estudo:
Estudo observacional
/
Fatores de risco
Limite:
Animais
/
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2004
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Fiocruz/BR
/
Instituto Oswaldo Cruz-Fiocruz/BR
/
Ministério da Agricultura, Pecuária e Abastecimento/BR
/
Universidade Federal de Minas Gerais/BR
/
Universidade Federal do Rio de Janeiro/BR
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