Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil
Mem. Inst. Oswaldo Cruz
;
99(8): 895-897, dez. 2004. tab
Artigo
em Inglês
| LILACS
| ID: lil-393775
ABSTRACT
The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9 percent), which were further characterized by nucleotide sequencing analysis. Of these, 60.9 percent were of HCV genotype 1 and 39.1 percent of genotype 3.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doadores de Sangue
/
Hepatite C
/
Hepacivirus
/
Anticorpos Anti-Hepatite C
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Agência Nacional de Vigilância Sanitária/BR
/
Hemocentro de Brasília/BR
/
Universidade Federal de Góias/BR
/
Universidade de Brasília/BR
Similares
MEDLINE
...
LILACS
LIS