High pressure-sensitive gene expression in Lactobacillus sanfranciscensis
Braz. j. med. biol. res
;
38(8): 1247-1252, Aug. 2005. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-405527
ABSTRACT
Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To investigate the mechanisms of the complex bacterial response to high pressure we have analyzed changes in the proteome and transcriptome by 2-D electrophoresis, and by microarrays and real time PCR, respectively. More than 16 proteins were found to be differentially expressed upon high pressure stress and were compared to those sensitive to other stresses. Except for one apparently high pressure-specific stress protein, no pressure-specific stress proteins were found, and the proteome response to pressure was found to differ from that induced by other stresses. Selected pressure-sensitive proteins were partially sequenced and their genes were identified by reverse genetics. In a transcriptome analysis of a redundancy cleared shot gun library, about 7 percent of the genes investigated were found to be affected. Most of them appeared to be up-regulated 2- to 4-fold and these results were confirmed by real time PCR. Gene induction was shown for some genes up-regulated at the proteome level (clpL/groEL/rbsK), while the response of others to high hydrostatic pressure at the transcriptome level seemed to differ from that observed at the proteome level. The up-regulation of selected genes supports the view that the cell tries to compensate for pressure-induced impairment of translation and membrane transport.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Regulação Bacteriana da Expressão Gênica
/
Pressão Hidrostática
/
Lactobacillus
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2005
Tipo de documento:
Artigo
/
Congresso e conferência
/
Documento de projeto
País de afiliação:
Alemanha
Instituição/País de afiliação:
Technische Universitãt München/DE
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