Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis
Mem. Inst. Oswaldo Cruz
;
100(2): 197-203, Apr. 2005. tab
Artigo
em Inglês
| LILACS
| ID: lil-410860
RESUMO
The performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a visceral leishmaniasis-endemic area in Brazil. Of 115 IFAT-reactive dogs at 140 titre, 106 (92.2 percent) were positive in parasitological exams (skin and/or spleen). These animals were compared to healthy animals (n = 25), negative for IFAT at a titre of 140 and parasitological exams. The sensitivities of crude and recombinant antigens were similar and remarkably high for both sera and eluates (97-100 percent). Specificity was higher than 96 percent for sera and eluates for different antigens, except for L. chagasi antigen using eluates (88 percent). Concordance values among the tests were higher either for sera or eluates (J = 0.95-1.00). High concordances were observed between sera and eluates tested with different antigens (kappa = 0.93-0.97). Crude and recombinant antigens identified different clinical phases of canine leishmaniasis. These results show that eluates could be used in canine surveys to identify L. chagasi infection. Recombinant antigens added little when compared to crude antigen in identifying positive dogs. Cross-reactivity with other diseases whose distribution often overlaps VL-endemic areas is a limitation of crude antigen use however.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Leishmania donovani
/
Ensaio de Imunoadsorção Enzimática
/
Doenças do Cão
/
Leishmaniose Visceral
/
Antígenos de Protozoários
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo prognóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2005
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Minas Gerais/BR
/
Universidade Federal de Ouro Preto/BR
Similares
MEDLINE
...
LILACS
LIS