Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV
RBCF, Rev. bras. ciênc. farm. (Impr.)
;
40(3): 373-380, jul.-set. 2004. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-420685
RESUMO
The oral dosages formulations from Pothomorphe umbellata (Piperaceae) can only be therapeutically evaluated after establishing 4-nerolidylcatechol (4-NRC) pharmacokinetic profile in plasma. For that purpose, a unique analytical method validation using HPLC-UV detection was developed for analysis of rat plasma samples. The animals received 10 mg/kg b.w. of 4-NRC i.v.. Analytical conditions were set with C18 column, methanol acetonitrile water (622018) as mobile phase, and a flow rate of 1.6 mL/min. The assay was linear from 1.0 - 80.0 mcg/mL. The recovery procedure was performed by liquid-liquid extraction showing quantitative extraction (106.4 ± 8.7 por cento)...
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DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plantas Medicinais
/
Tecnologia Farmacêutica
/
Medicina Herbária
Limite:
Animais
Idioma:
Inglês
Revista:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Assunto da revista:
Bioquímica
/
Farmácia
/
Farmacologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR
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