Evaluation of the dot enzyme-linked immunosorbent assay in comparison with standard ELISA for the immunodiagnosis of human toxocariasis
Mem. Inst. Oswaldo Cruz
;
101(1): 71-74, Feb. 2006.
Artigo
em Inglês
| LILACS
| ID: lil-423565
ABSTRACT
A dot enzyme-linked immunosorbent assay (dot-ELISA) was standardized using excretory-secretory antigens of Toxocara canis for the rapid immunodiagnosis of human toxocariasis. Thirty patients with clinical signs of toxocariasis, 20 cases with other parasitic diseases, and 40 healthy subjects were tested. A total of 0.2 ng of antigen per dot, serum dilution of 1160 and dilution conjugate of 11000 were found optimal. The sensitivity and specificity of the assay were 100 and 95 percent, respectively. Comparable sensitivity of dot-ELISA and the standard ELISA was obtained, but only 3 cross-reactions occurred in the dot-ELISA, compared with 6 in the standard ELISA. Dot-ELISA is simple to perform, rapid, and low cost. Large-scale screening studies should be done to evaluate its usefulness under field conditions.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
/
Toxocaríase
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo prognóstico
/
Fatores de risco
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2006
Tipo de documento:
Artigo
País de afiliação:
Peru
Instituição/País de afiliação:
Universidad Nacional Mayor de San Marcos/PE
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