Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
Rev. Soc. Bras. Med. Trop
;
39(2): 159-162, mar.-abr. 2006. ilus, tab
Artigo
em Inglês
| LILACS, SES-SP
| ID: lil-426908
ABSTRACT
A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100 percent agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Raiva
/
Vírus da Raiva
/
DNA Viral
/
Digoxigenina
/
Nucleoproteínas
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Ano de publicação:
2006
Tipo de documento:
Artigo
Instituição/País de afiliação:
Institute Pasteur São Paulo/BR
/
University of São Paulo/BR
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