Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
Biol. Res
;
39(1): 25-37, 2006. tab
Artigo
em Inglês
| LILACS
| ID: lil-430695
ABSTRACT
Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during post-natal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duodenal and jejunal mucosa of three groups each of control and iron-deficient rats and hybridized with RAE 230A and 230B gene chips (Affymetrix). Stringent data reduction strategies were employed. Results showed that several genes were similarly induced in both gut segments, including DMT1, Dcytb, transferrin receptor 1, heme oxygenase 1, metallothionein, the Menkes copper ATPase (ATP7A), tripartitie motif protein 27, and the sodium-dependent vitamin C transporter. However, a subset of genes showed regulation in only one or the other gut segment. In duodenum only, gastrokine 1, trefoil factor 1 and claudin 2 were induced by iron-deficiency. Other genes previously identified were only regulated in the duodenum. Overall, these studies demonstrate similarities and distinct differences in the genetic response to iron deprivation in the duodenum versus jejunum and provide evidence that more distal gut segments also may play a role in increasing iron absorption in iron-deficiency anemia.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Análise de Sequência com Séries de Oligonucleotídeos
/
Duodeno
/
Absorção Intestinal
/
Mucosa Intestinal
/
Ferro
/
Jejuno
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2006
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Estados Unidos
Instituição/País de afiliação:
University of Arizona/US
Similares
MEDLINE
...
LILACS
LIS