Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
Biol. Res
;
39(2): 269-279, 2006. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-432429
RESUMO
Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg, even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infection.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doenças das Plantas
/
Nicotiana
/
Vírus do Mosaico do Tabaco
/
Plantas Geneticamente Modificadas
/
Proteínas do Movimento Viral em Plantas
/
Mutação
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2006
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
/
Estados Unidos
Instituição/País de afiliação:
Harvard Medical School/US
/
Pontificia Universidad Católica de Chile/CL
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