Entamoeba histolytica and Entamoeba dispar: differentiation by Enzyme-Linked Immunosorbet Assay (ELISA) and its clinical correlation in pediatric patients

ABSTRACT

Studies were carried out at a mexican pediatric hospital to determine the ratio between the pathogenic species Entamoeba histolytica and non-pathogenic species E. dispar using an enzyme-linked immunosorbent assay (ELISA) to detect the lectin (1 galactose N-acetyl D-galactosamine) of E. histolytica in feces. A close correlation was noted between the presence of the E. histolytica lectin and clinical symptoms. In the study, amebas were detected by microscopy in 120 children (either E. histolytica or E. dispar). But while almost all (13/14) of the children with E. histolytica had clinical symptoms, dysentery-feces with mocus and blood, diarrhea, cramping abdominal pain, tenesmus rectal, flatulence, vomiting and headache, almost none (1/106) of the children infected with the non-pathogenic ameba E. dispar had signs and symptoms. This suggests that much of the amebiasis diagnoses made in Mexico are, in fact, due to non-pathogenic E. dispar.