Cyclosporin A in skin samples from in vitro penetration studies may be assayed by a simple HPLC method
RBCF, Rev. bras. ciênc. farm. (Impr.)
;
41(4): 477-482, out.-dez. 2005. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-433325
RESUMO
A simple High Perfomance Liquid Chromatography method with UV detection was developed for quantification of Cyclosporin A (CysA) in skin sample after in vitro penetration studies. The peptide was recovered from two different layers of skin, the stratum corneum and the epidermis plus dermis ([E+D]), by vortex homogenization and bath sonication in an organic solvent (methanol). Recovery of CysA from skin was about 85 percent and CysA was estimated by HPLC using a RP-18 column, UV detection at 210 nm and acetronitrile water (6733 v/v) as the mobile phase. The quantification limit was 105ng/mL. The assay was linear from 0.15-500 µg/mL. The within-day and between-day assay precision and accuracy were studied at three concentration levels (1, 10 and 20 µg/mL)...
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Dermatopatias
/
Técnicas In Vitro
/
Ciclosporina
Idioma:
Inglês
Revista:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Assunto da revista:
Bioquímica
/
Farmácia
/
Farmacologia
Ano de publicação:
2005
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR
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