Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama
Mem. Inst. Oswaldo Cruz
;
101(4): 455-457, June 2006. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-435309
ABSTRACT
The present work provides information on Trypanosoma cruzi genotype circulating in endemic areas of Chagas disease in Panama. A total of 26 crude stocks of T. cruzi, isolated from the blood of persons with different clinical profiles of Chagas disease were collected and crio-conserved until used. Most of the stocks had been characterized by means of isoenzyme electrophoresis on cellulose acetate membranes. The clinical profiles of infected persons included 9 (34.6 percent) asymptomatic and 17 acute (65.4 percent) including 5 (19.2 percent) fatal cases, 2 under 5 years old and 3 adults. A multiplex-PCR assay based on the amplification of the non-transcribed spacer of the mini-exon gene was performed. All stocks of T. cruzi included in the study were found to correspond to Tc I group. This result supports the predominance of T. cruzi-I in the transmission cycles affecting the human population in the Republic of Panama.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Trypanosoma cruzi
/
Doença de Chagas
/
Doenças Endêmicas
Limite:
Animais
/
Humanos
País/Região como assunto:
América Central
/
Panamá
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2006
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Panamá
Instituição/País de afiliação:
Instituto Conmemorativo Gorgas de Estudios de la Salud/PA
/
University of Panama/PA
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