Imaging single-channel calcium microdomains by total internal reflection microscopy
Biol. Res
;
37(4): 675-679, 2004. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-437525
ABSTRACT
The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Canais de Cálcio
/
Trocador de Sódio e Cálcio
/
Canais Iônicos
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Estados Unidos
Instituição/País de afiliação:
University of California/US
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