Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos / Genotypying of clinical isolates of Helicobacter pylori by cagA, vacA and babA2 virulence associated genes: First detection of a babA2 positive strain in Chilean patients
Rev. méd. Chile
;
134(8): 981-988, ago. 2006. ilus, tab, graf
Artigo
em Espanhol, Inglês
| LILACS
| ID: lil-438368
ABSTRACT
Background:
Helicobacter pylori-associated gastroduodenal diseases depends on host characteristics, environmental conditions and bacterial virulence factors, such as cagA, vacA y babA2 gene products. Moreover, peptic ulcer disease has been related with cagA+, vacAs1m1 strains, while metaplasia and gastric cancer has been associated to cagA+, vacAs1 and babA2+ H pylori strains. Gene babA2 has not yet been described in clinical isolates from Chilean patients.Aim:
To investigate the presence of cagA, vacA (s and m) and babA2 genes in clinical isolates of H pylori from Chilean patients. Material andMethods:
Sixty six isolates from 41 patients were genotyped by PCR, using primers for s1a, s1b, s2, m1, m2, cagA and babA2 genes as previously described.Results:
cagA gene was detected in 16 isolates (24.2 percent) while vacAs1a, vacAs1b, vacAs2, vacAm1 and vacAm2 were detected in 28 (42.4 percent), 14 (21.2 percent), 17 (25.8 percent), 21 (31.8 percent) and 29 isolates (43.9 percent), respectively. One isolate (1.5 percent) was babA2 positive, being the first isolate with this genotype described in Chile. Besides the babA2+ genotype this clinical isolate also presented cagA+ and vacAs1a which has been related with metaplasia or gastric cancer. Five isolates showed an ulcerogenic profile cagA+, vacAs1m1.Conclusions:
The results presented indicate the prevalence of vacAs1m1 genotype among the clinical isolates analyzed, and a low frequency of babA2 genotype.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Proteínas de Bactérias
/
Helicobacter pylori
/
Infecções por Helicobacter
/
Adesinas Bacterianas
/
Gastroenteropatias
/
Antígenos de Bactérias
Tipo de estudo:
Estudo diagnóstico
/
Fatores de risco
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Chile
Idioma:
Inglês
/
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2006
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
/
Estados Unidos
Instituição/País de afiliação:
Ochsner Clinic Foundation/US
/
Universidad de Concepción/CL
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