In silico evaluation of the Eucalyptus transcriptome
Genet. mol. biol
;
28(3,suppl): 487-495, Nov. 2005. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-440443
ABSTRACT
The expressed sequence tags (ESTs) produced in the Forests project provide an invaluable opportunity to assess the Eucalyptus transcriptome. Besides providing information on the different proteins produced by this plant, it is possible to infer gene expression profiles because non-normalized cDNA libraries were used. The EST frequency from any gene is correlated to the transcript levels in the tissues from which the cDNA libraries were constructed. The goal of this work was to identify Eucalyptus genes that showed either differential expression pattern or were ubiquitously expressed in the tissues sampled in the Forests project. Six robust statistical tests and very restrictive rules were applied to gain confidence in the in silico data aiming to avoid false positives. Several genes with interesting expression profiles were identified and some of them were validated by RT-PCR
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plantas
/
Expressão Gênica
/
Eucalyptus
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2005
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual Paulista "Júlio Mesquita Filho"/BR
/
Universidade Estadual de Campinas/BR
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