Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients
Mem. Inst. Oswaldo Cruz
;
101(8): 857-861, Dec. 2006. tab
Artigo
em Inglês
| LILACS
| ID: lil-440572
ABSTRACT
Human pulmonary tuberculosis (TB) is a worldwide public health problem. In resistant individuals, control of the infection mainly requires development of a Th1 cell immune response with production of cytokines, of which interferon-gamma (IFN-gamma)plays an important role. Several antigens from Mycobacterium tuberculosis complex has been described for use in vaccine development or for diagnostic purposes, however little evaluation has been done in endemic area for TB. The proliferative and IFN-gamma human T cell immune responses, to four recombinant proteins (MBP-3, NarL, MT-10.3, 16 kDa) and PPD, of 38 Brazilian TB patients (6 untreated and 32 treated) and 67 controls (38 positive and 29 negative tuberculin skin test - TST) were compared. The highest reactivity mean rate was obtained with PPD followed by 16 kDa in TB patients. While most of the patients (87 percent) and controls (> 64 percent) respond to the PPD, 16kDa was more specifically recognized (> 21 percent) although less sensitive (54 percent). When TB patients were divided according to treatment status, opposite to PPD, higher average level of IFN-gamma was induced by 16kDa in untreated (505 pg/ml) compared to treated TB patients and TST+ (269.8 pg/ml x 221.6pg/ml, respectively), although the difference was not significant. These data show that in contrast with the other recombinant proteins, the stimulatory potency of 16kDa to induce proliferative and INF-gamma response was more effective and is more recognized by active TB untreated patients, eliciting in control individuals a more selective immune response than PPD.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Tuberculose Pulmonar
/
Proteínas Recombinantes
/
Leucócitos Mononucleares
/
Interferon gama
/
Proliferação de Células
/
Antígenos de Bactérias
Tipo de estudo:
Estudo observacional
Limite:
Feminino
/
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2006
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
/
Alemanha
Instituição/País de afiliação:
Fundacao Oswaldo Cruz/BR
/
Hospital Municipal Souza Aguiar/BR
/
Lionex Immuno Diagnostic and Therapeutic/DE
/
Universidade Federal do Rio de Janeiro/BR
Similares
MEDLINE
...
LILACS
LIS