TcZFP8, a novel member of the Trypanosoma cruzi CCHC zinc finger protein family with nuclear localization
Genet. mol. res. (Online)
;
5(3): 553-563, 2006. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-441051
ABSTRACT
In a 17-kb genomic fragment of Trypanosoma cruzi chromosome XX, we identified three tandemly linked genes coding for CX2CX4HX4C zinc finger proteins. We also showed that similar genes are present in T. brucei and Leishmania major, sharing three monophyletic groups among these trypanosomatids. In T. cruzi, TcZFP8 corresponds to a novel gene coding for a protein containing eight zinc finger motifs. Molecular cloning of this gene and heterologous expression as a fusion with a His-tag were performed in Escherichia coli. The purified recombinant protein was used to produce antibody in rabbits. Using Western blot analysis, we observed the presence of this protein in all three forms of the parasite: amastigote, trypomastigote and epimastigote. An analysis of cytoplasmic and nuclear cell extracts showed that this protein is present in nuclear extracts, and indirect immunofluorescence microscopy confirmed the nuclear localization of TcZFP8. Homologues of TcZFP8 in T. brucei are apparently absent, while one candidate in L. major was identified.
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Índice:
LILACS (Américas)
Assunto principal:
Trypanosoma cruzi
/
Proteínas de Protozoários
/
Núcleo Celular
/
Dedos de Zinco
/
Código Genético
Limite:
Animais
Idioma:
Inglês
Revista:
Genet. mol. res. (Online)
Assunto da revista:
Biologia Molecular
/
Genética
Ano de publicação:
2006
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de Brasília/BR
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