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A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
Adema, Coen M; Luo, Mei-Zhong; Hanelt, Ben; Hertel, Lynn A; Marshall, Jennifer J; Zhang, Si-Ming; Dejong, Randall J; Kim, Hye-Ran; Kudrna, David; Wing, Rod A; Soderlund, Cari; Knight, Matty; Lewis, Fred A; Caldeira, Roberta Lima; Jannotti-Passos, Liana K; Carvalho, Omar dos Santos; Loker, Eric S.
  • Adema, Coen M; University of New Mexico. Department of Biology. Albuquerque. US
  • Luo, Mei-Zhong; Arizona Genomics Institute. Department of Plant Sciences. Arizona. US
  • Hanelt, Ben; University of New Mexico. Department of Biology. Albuquerque. US
  • Hertel, Lynn A; s.af
  • Marshall, Jennifer J; University of New Mexico. Department of Biology. Albuquerque. US
  • Zhang, Si-Ming; University of New Mexico. Department of Biology. Albuquerque. US
  • Dejong, Randall J; University of New Mexico. Department of Biology. Albuquerque. US
  • Kim, Hye-Ran; Arizona Genomics Institute. Department of Plant Sciences. Arizona. US
  • Kudrna, David; Arizona Genomics Institute. Department of Plant Sciences. Arizona. US
  • Wing, Rod A; Arizona Genomics Institute. Department of Plant Sciences. Arizona. US
  • Soderlund, Cari; University of Arizona. BIO5 Institute. Arizona Genomics Computational Laboratory. Tucson. US
  • Knight, Matty; Biomedical Research Institute. Rockville. US
  • Lewis, Fred A; Biomedical Research Institute. Rockville. US
  • Caldeira, Roberta Lima; Fiocruz. Centro de Pesquisas René. Laboratório de Helmintoses Intestinais. Belo Horizonte. BR
  • Jannotti-Passos, Liana K; Fiocruz. Centro de Pesquisas René. Laboratório de Helmintoses Intestinais. Belo Horizonte. BR
  • Carvalho, Omar dos Santos; Fiocruz. Centro de Pesquisas René. Laboratório de Helmintoses Intestinais. Belo Horizonte. BR
  • Loker, Eric S; University of New Mexico. Department of Biology. Albuquerque. US
Mem. Inst. Oswaldo Cruz ; 101(supl.1): 167-177, Oct. 2006. tab, graf
Artigo em Inglês | LILACS | ID: lil-441243
ABSTRACT
To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html">biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains ~ 63 percent AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.
Assuntos
Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Schistosoma mansoni / Biomphalaria / Biblioteca Gênica / Cromossomos Artificiais Bacterianos Limite: Animais Idioma: Inglês Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: Medicina Tropical / Parasitologia Ano de publicação: 2006 Tipo de documento: Artigo / Documento de projeto País de afiliação: Brasil / Estados Unidos Instituição/País de afiliação: Arizona Genomics Institute/US / Biomedical Research Institute/US / Fiocruz/BR / University of Arizona/US / University of New Mexico/US

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Schistosoma mansoni / Biomphalaria / Biblioteca Gênica / Cromossomos Artificiais Bacterianos Limite: Animais Idioma: Inglês Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: Medicina Tropical / Parasitologia Ano de publicação: 2006 Tipo de documento: Artigo / Documento de projeto País de afiliação: Brasil / Estados Unidos Instituição/País de afiliação: Arizona Genomics Institute/US / Biomedical Research Institute/US / Fiocruz/BR / University of Arizona/US / University of New Mexico/US