Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni
Mem. Inst. Oswaldo Cruz
;
101(supl.1): 355-357, Oct. 2006. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-441274
ABSTRACT
The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Peptídeos
/
Esquistossomose mansoni
/
Proteínas de Helminto
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2006
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Estados Unidos
Instituição/País de afiliação:
Center for Biologics Evaluation and Research/US
/
Universidade de São Paulo/BR
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