Evaluation of photodynamic activity of octaethylporphyrin and vanadyl octaethylporphyrin
Appl. cancer res
;
25(3): 142-150, July-Sept. 2005.
Artigo
em Inglês
| LILACS, Inca
| ID: lil-442308
ABSTRACT
Objective:
in this work we have investigated the photodynamic efficiency of octaethylporphyrin (OEP) and vanadyl octaethylporphyrin(VOOEP).Methods:
this study was performed by the evaluation of photophysical parameters of these porphyrins, the photooxidationrate constants (kf) of the biomolecules (tryptophan -Trp and bovine serum albumin - BSA) and the erythrocytes photodestructionpercentage.Results:
photophysical parameters value such as singlet oxygen quantum yield (Õ∆) and triplet state lifetime (ôT)indicated that OEP (Õ∆= 0.64 ±0.02, tT= 0.91 ± 0.02 ms) is more efficient than VOOEP (Õ∆= 0.26 ±0.02, tT= 0.22 ± 0.03ms). The values of kf/10-4 s-1for Trp and BSA photoxidation demonstrated that OEP (Trp= 2.80 ± 0.05 and BSA= 2.50 ± 0.1)is more efficient than VOOEP (Trp= 0.81 ± 0.08 and BSA= 0.62 ± 0.04). The photodestruction percentage of erythrocytesrevealed that the photodynamic activity of OEP is more pronounced than photoactivity of VOOEP. These results indicated thatdifferences observed in the photodynamic activity between the porphyrins could be associated with differences in their molecularstructures.Conclusion:
photophysical parameters, photooxidation of biomolecules, and photodestruction of erythrocytes clearlyindicate that the vanadyl group (V=O) interferes in the photoactivity of OEP, causing a considerable reduction in its efficiency.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fotoquimioterapia
/
Porfirinas
/
Hemólise
Idioma:
Inglês
Revista:
Appl. cancer res
Assunto da revista:
Neoplasias
Ano de publicação:
2005
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Campinas/BR
/
Universidade de São Paulo/BR
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