Your browser doesn't support javascript.
loading
Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment
Tamieti, B. P; Damatta, R. A; Cogo, J. C; Da Silva, N. S; Mittmann, J; Pacheco-Soares, C.
  • Tamieti, B. P; Vale do Paraíba University. Institute of Research and Development. Laboratory of Cell and Tissue Biology. São José dos Campos. BR
  • Damatta, R. A; State University of Norte Fluminense. Center for Biosciences and Biotechnology. Laboratory of Cell and Tissue Biology. Campos dos Goytacazes. BR
  • Cogo, J. C; Vale do Paraíba University. Institute of Research and Development. Laboratory of Physiology and Pharmacology. São José dos Campos. BR
  • Da Silva, N. S; Vale do Paraíba University. Institute of Research and Development. Laboratory of Cell and Tissue Biology. São José dos Campos. BR
  • Mittmann, J; Vale do Paraíba University. Institute of Research and Development. Laboratory of Molecular Biology and Immunobiologics. São José dos Campos. BR
  • Pacheco-Soares, C; Vale do Paraíba University. Institute of Research and Development. Laboratory of Cell and Tissue Biology. São José dos Campos. BR
J. venom. anim. toxins incl. trop. dis ; 13(1): 56-68, 2007. ilus
Artigo em Inglês | LILACS | ID: lil-444611
ABSTRACT
Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.
Assuntos
Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Células CHO / Venenos de Crotalídeos / Retículo Endoplasmático Limite: Animais Idioma: Inglês Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: Toxicologia Ano de publicação: 2007 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: State University of Norte Fluminense/BR / Vale do Paraíba University/BR

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Células CHO / Venenos de Crotalídeos / Retículo Endoplasmático Limite: Animais Idioma: Inglês Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: Toxicologia Ano de publicação: 2007 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: State University of Norte Fluminense/BR / Vale do Paraíba University/BR