Plant regeneration of Anthurium andreanum cv Rubrun
Electron. j. biotechnol
;
7(3): 10-11, Dec. 2004. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-448767
ABSTRACT
To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 muM BA. After 2 weeks, 74 percent of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 muM BA and 0.05 muM NAA. On average, 3.6 shoots per explant were obtained. Four weeks old in vitro plants from germinated seeds and the plantlets obtained from micro-cuttings, showed callus proliferation at the stem base. These tissues were subcultured on a medium supplemented with 8.9 muM BA and 2.7 muM NAA. After 6 weeks of culture, about 43.8 plantlets per square cm of callus were obtained. Anatomical studies showed the organogenic nature of these calli. Anthurium andreanum plants regenerated by organogenesis were transferred to pots and a rate of 80 percent of plant acclimatization was obtained.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Araceae
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Venezuela
Instituição/País de afiliação:
Universidad Central de Venezuela/VE
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