Preliminary results of using ESR to examine biofilms
Electron. j. biotechnol
;
7(3): 11-12, Dec. 2004. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-448768
ABSTRACT
This preliminary work shows ESR (Electron Spin Resonance) can be used to detect biofilms, particularly from Fe-metabolising bacteria. A film was detected by ESR as early as 1 day, hence possibly more sensitively than by fluorescent methods. Films can probably be detected as early as one hour. Spectra contain a very broad peak at g=2.13, probably due to ferrihydrite. Results of field experiments from streams and ponds in New Zealand and Japan, particularly the Minoh River, showed a general increase of ferrihydrite with time. Loss by exfoliation was later than 20 days. The rate of accumulation was faster in a nutrient-rich stagnant pond. Hematite (g=4.3) was often observed, magnetite (g=9) once, and usually small amounts of a common bacterial decay product. The latter was detected for at least 18 months film storage. ESR is a particularly good tool for observing the growth of oxic biofilms containing Fe-metabolising bacteria, and should be just as sensitive for observing Mn-metabolising bacteria in reducing conditions.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Bactérias
/
Compostos Férricos
/
Espectroscopia de Ressonância de Spin Eletrônica
/
Biofilmes
/
Óxido Ferroso-Férrico
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Japão
Instituição/País de afiliação:
Kanazawa University/JP
/
Osaka University/JP
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