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Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites
Pérez de la Torre, Mariana C; Escandón, Alejandro S.
  • Pérez de la Torre, Mariana C; INTA Castelar. Instituto de Floricultura. Buenos Aires. AR
  • Escandón, Alejandro S; INTA Castelar. Instituto de Floricultura. Buenos Aires. AR
Electron. j. biotechnol ; 9(3)June 2006. tab
Artigo em Inglês | LILACS | ID: lil-448820
ABSTRACT
The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98 percent of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular identification profile for the six new varieties. This result, supported together with our previous reports, indicates that the anchored microsatellites are a very useful technique for the fingerprints generation in N. linariaefolia.
Texto completo: DisponíveL Índice: LILACS (Américas) Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2006 Tipo de documento: Artigo / Documento de projeto País de afiliação: Argentina Instituição/País de afiliação: INTA Castelar/AR

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Texto completo: DisponíveL Índice: LILACS (Américas) Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2006 Tipo de documento: Artigo / Documento de projeto País de afiliação: Argentina Instituição/País de afiliação: INTA Castelar/AR