Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil
Braz. j. infect. dis
;
11(1): 27-30, Feb. 2007. tab
Artigo
em Inglês
| LILACS
| ID: lil-454679
ABSTRACT
Genetic analysis of HIV-1 is essential to improve treatment strategies and select epitopes for vaccine programs. The objective of this study was to determine whether known CD4+ and CD8+ epitopes were present in Brazilian HIV-1 strains. We used previously described CD8+ and CD4+ epitopes from the Los Alamos laboratory to search for these epitopes in the Brazilian sequences using the HIVbase program and we compared the frequency results with the analyses using physical-chemical profile tools from Network Protein Sequence Analysis (NPSA), and the SYFPEITHI program. Furthermore, this analysis was carried out with the Prosite tool using the GeneDoc program and ds/dn analyses using the Synonymous Nonsynonymous Analysis Program (SNAP). The HIVbase epitope mapping demonstrated that 30 CD8+ and 6 CD4+ epitopes were present in the Brazilian sequences at a high frequency. Only two of these epitopes were heavily glycosylated. Interestingly, ds/dn analyses showed evidence of purifying selective pressure. These types of analyses could be useful for the assessment of possible vaccine efficiency in populations.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Produtos do Gene env
/
Infecções por HIV
/
HIV-1
/
Epitopos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. infect. dis
Assunto da revista:
Doenças Transmissíveis
Ano de publicação:
2007
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
/
Estados Unidos
/
Reino Unido
Instituição/País de afiliação:
Oswaldo Cruz Foundation/BR
/
University of Oxford/GB
/
University of Wisconsin Medical School/US
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