Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
Biol. Res
;
40(1): 85-92, 2007. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-456611
ABSTRACT
This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Photobacterium
/
Variação Genética
/
Impressões Digitais de DNA
/
Técnicas de Tipagem Bacteriana
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2007
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Itália
/
Espanha
Instituição/País de afiliação:
CNR Sezione di Messina/IT
/
Universidad de Santiago de Compostela/ES
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