Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
Mem. Inst. Oswaldo Cruz
;
102(5): 587-592, Aug. 2007. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-458626
ABSTRACT
Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O1a and O3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3 percent genetic similarity, ribotypes 2 and 3 presented 52.5 percent genetic similarity, and genetic similarity was 45 percent between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plasmídeos
/
Yersinia pseudotuberculosis
/
Ribotipagem
/
Fatores de Virulência
/
Ilhas Genômicas
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2007
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual Paulista/BR
/
Universidade de Franca/BR
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