Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo)
Mem. Inst. Oswaldo Cruz
;
102(5): 639-642, Aug. 2007. tab
Artigo
em Inglês
| LILACS
| ID: lil-458627
ABSTRACT
Mycobacterium was verified in animals from a Brazilian dairy herd, a total of 42 samples from 30 cows were submitted to culture and the isolated strains were analyzed by two polymerase chain reaction (PCR), the first specific for species belonging to the Mycobacterium complex (MTBC) and the other for differentiating M. tuberculosis from M. bovis. Twenty seven samples (64.3 percent) from 18 animals (60 percent) were positive for mycobacteria by culture, including samples from 15 retrofaryngeal lymphnodes (55.5 percent), 9 prescapular lymphnodes (33.3 percent), 2 lungs (7.4 percent), and 1 liver (3.7 percent). All isolated colonies were confirmed by PCR to contain MTBC organisms, and were identified as M. bovis by the same methodology.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Tuberculose Bovina
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2007
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Coordenadoria de Assistência Técnica Integral/BR
/
Instituto Biológico/BR
/
Universidade de São Paulo/BR
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