The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
Biol. Res
;
40(2): 185-192, 2007. ilus
Artigo
em Inglês
| LILACS
| ID: lil-468189
ABSTRACT
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fatores de Transcrição
/
Cromatina
/
Ciclo Celular
/
Replicação do DNA
Tipo de estudo:
Guia de Prática Clínica
Limite:
Humanos
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2007
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Alemanha
Instituição/País de afiliação:
Federal Agricultural Research Centre/DE
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