Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato
Mem. Inst. Oswaldo Cruz
;
102(8): 999-1002, Dec. 2007. ilus
Artigo
em Inglês
| LILACS
| ID: lil-471853
ABSTRACT
The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Grupo Borrelia Burgdorferi
/
Meios de Cultura
Tipo de estudo:
Estudos de avaliação
/
Pesquisa qualitativa
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2007
Tipo de documento:
Artigo
País de afiliação:
Cuba
/
Suíça
Instituição/País de afiliação:
ADMed/CH
/
Tropical Medicine Institute Pedro Kourí/CU
/
University of Neuchâtel/CH
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