P53 gene mutation and protein expression in ameloblastomas
Braz. j. oral sci
;
5(17): 1034-1040, 2006. ilus, tab, graf
Artigo
em Inglês
| LILACS, BBO
| ID: lil-472515
ABSTRACT
P53 gene is commonly found to be mutated in human cancer. Currently, limited data exist on the occurrence of p53 gene mutation in ameloblastomas. This study designed to evaluate the expression of p53 proteins and their gene mutations in human ameloblastomas. Four cases of epithelial odontogenic tumors were used. Normal cells of a 7 months aborted fetuss mandible were used as negative control. P53 Protein expression was detected immunohistochemically. DNA was extracted and amplified using PCR. Gene sequences analyzed to determine p53 mutation. p53 was overexpressed in one case of unicystic ameloblastoma and 2 cases of variant types of ameloblastoma. The Coding sequencing analyses demonstrated p53 gene alteration in cases of ameloblastoms in Exon 7 while, fetus epithelial mucosal cells showed the wild type DNA sequence in Exon 4. In conclusion p53 protein overexpression was detected by immunohistochemical staining as well as by DNA sequence analysis in cases of ameloblastomas.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Imuno-Histoquímica
/
Ameloblastoma
/
Mandíbula
Idioma:
Inglês
Revista:
Braz. j. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2006
Tipo de documento:
Artigo
País de afiliação:
Malásia
Instituição/País de afiliação:
Universiti Sains Malaysia/MY
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