Evaluation of a malaria antibody enzyme immunoassay for use in blood screening
Mem. Inst. Oswaldo Cruz
;
103(1): 75-78, Feb. 2008. tab
Artigo
em Inglês
| LILACS
| ID: lil-478882
ABSTRACT
Transfusion-transmitted malaria is rare, but it may produce severe problem in the safety of blood transfusion due to the lack of reliable procedure to evaluate donors potentially exposed to malaria. Here, we evaluated a new enzyme-linked immunosorbent assay malaria antibody test (ELISA malaria antibody test, DiaMed, Switzerland) to detect antibodies to Plasmodium vivax (the indigenous malaria) in the blood samples in the Republic of Korea (ROK). Blood samples of four groups were obtained and analyzed; 100 samples from P.vivax infected patients, 35 from recovery patients, 366 from normal healthy individuals, and 325 from domestic travelers of non-endemic areas residents to risky areas of ROK. P.vivax antibody levels by ELISA were then compared to the results from microscopic examination and polymerase chain reaction (PCR) test. As a result, the ELISA malaria antibody test had a clinical sensitivity of 53.0 percent and a clinical specificity of 94.0 percent for P.vivax. Twenty out of 325 domestic travelers (6.2 percent) were reactive and 28 cases (8.6 percent) were doubtful. Of the reactive and doubtful cases, only two were confirmed as acute malaria by both microscopy and PCR test. Thus we found that the ELISA malaria antibody test was insufficiently sensitive for blood screening of P.vivax in ROK.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plasmodium vivax
/
Doadores de Sangue
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Antiprotozoários
/
Malária Vivax
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo observacional
/
Estudo prognóstico
/
Fatores de risco
/
Estudo de rastreamento
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Coréia do Sul
Instituição/País de afiliação:
Korea University Ansan Hospital/KR
/
Korea University/KR
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