Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability
Mem. Inst. Oswaldo Cruz
;
103(2): 172-179, Mar. 2008. graf, ilus
Artigo
em Inglês
| LILACS
| ID: lil-480645
ABSTRACT
Rhodnius prolixus is the main Trypanosoma rangeli vector in several Latin-American countries and is susceptible to infection with KP1(+) strains; however, it presents an invasion-resistant response to KP1(-) strains. The present work has identified a trypanolytic protein against T. rangeli KP1(-) in the R. prolixus hemolymph which was fractioned with ammonium sulfate (following dialysis). The results revealed a protein component which did not depend on divalent cations for its biological function whilst keeping its trypanolytic activity at temperatures ranging from -20ºC to 37ºC, at 7.0 to 10.5 pH. The protein was partially purified by gel filtration chromatography and ionic exchange chromatography. The major component presented a molecular weight of around 79 kDa and an isoelectric point between 4.9 and 6.3 and may be directly related to hemolymph trypanolytic activity against T. rangeli KP1(-) populations.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Rhodnius
/
Trypanosoma
/
Hemolinfa
/
Proteínas de Protozoários
/
Insetos Vetores
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2008
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Colômbia
Instituição/País de afiliação:
Universidad Nacional de Colombia/CO
/
Universidad del Tolima/CO
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