Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa
Genet. mol. res. (Online)
;
5(4): 851-855, 2006. ilus
Artigo
em Inglês
| LILACS
| ID: lil-482073
ABSTRACT
DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yield from the hyphae of Crinipellis perniciosa, which causes witches' broom disease in cacao, from three other filamentous fungi, Lentinus edodes, Agaricus blazei, Trichoderma stromaticum, and from the yeast S. cerevisiae. Genomic DNA was suitable for PCR of specific actin primers of C. perniciosa, allowing it to be differentiated from fungal contaminants, including its natural competitor, T. stromaticum.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
DNA Fúngico
/
Técnicas de Tipagem Micológica
/
Genoma Fúngico
/
Agaricales
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Genet. mol. res. (Online)
Assunto da revista:
Biologia Molecular
/
Genética
Ano de publicação:
2006
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Santa Cruz/BR
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