Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana / Millerenolide induces suppressive activity on human peripheral blood mononuclear cells
Rev. méd. Chile
;
136(1): 64-72, ene. 2008. ilus, graf
Artigo
em Espanhol
| LILACS
| ID: lil-483221
ABSTRACT
Background: Natural products are used in the production of therapeutic drugs due to their wide diversity and excellent adaptability to biological structures. Sesquiterpene ¡aciones are the active constituents of several plants from the Asteraceae family. Aim: To assess the in vitro effect of a sesquiterpene lactone (millerenolide). Material and methods: The drug effect was assessed measuring the proliferation of lymphocytes using the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonylJ-2H-tetrazolium hydroxide (XTT) technique. Changes on the cell cycle were analyzed on a FACSort flow cytometer The effect of millerenolide on the production of nitric oxide (NO) by macrophages was evaluated using the Griess reagent. Additionally, phagocytosis of latex particles and nitroblue tetrazolium (NBT) reduction by macrophages were evaluated microscopically. Results: Treatment of human peripheral blood mononuclear cells (PBMC) with millerenolide decreases the proliferation of lymphocytes, decreases the percentage of cells in S, and G2/Mphases, and increases the proportion of cells in GO/Gl phase. Treatment of macrophages with millerenolide, reduces the production of NO, the phagocytic capacity and the number of cells able to reduce NBT. Cytotoxic effects of the lactone on human PBMC were only observed when the concentration was increased to 6 fig/ml. Conclusions: Millerenolide could be considered as a potential therapeutic agent with immunosuppressiveproperties.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Sesquiterpenos
/
Técnicas In Vitro
/
Leucócitos Mononucleares
/
Ativação Linfocitária
/
Imunossupressores
/
Lactonas
/
Óxido Nítrico
Limite:
Humanos
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Costa Rica
Instituição/País de afiliação:
Centro para Investigaciones en Biología Celular y Molecular/CR
/
Universidad de Costa Rica/CR
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